Nettet13. mar. 2024 · Mouse ES(embryonic stem) Cell Nucleofector® Kit配有专门的操作手册,试剂盒内除含有特异的核转染液(Nucleofector™溶液),另外含有pmaxGFP™对照 … NettetKit components 2.25 ml Mouse ES Cell Nucleofector® Solution 0.5 ml Supplement 10 µg pmaxGFP® (0.5 g/ l in 10 mM Tris pH 8.0) 25 certified cuvettes 25 plastic pipettes Size …
Amaxa® Cell Line Nucleofector® Kit V - Picturepark
Nettet3. jun. 2024 · P3 Primary Cell 4D-Nucleofector X Kit S: Lonza: V4XP-3032: FBS: MSK: N/A: Glasgow Minimum Essential Media: Sigma: G5154: Proteinase K: Roche: … Nettetfor Mouse ES Cells Product Description Recommended Kits P3 Primary Cell 96-well Nucleofector™ Kits Cat. No. V4SP-3096 Size (reactions) 1×96 P3 Primary Cell 96-well Nucleofector™ Solution 2.25 ml Supplement 0.5 ml pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0) 50 μg Nucleocuvette™ Plate(s) 1 Cat. No. V4SP-3960 revoir je te promets
Mouse T Cell Nucleofector™ Kit Lonza
NettetProduct Overview. The P3 Primary Cell 4D-Nucleofector TM X Kit S is one of our five 4D-Nucleofector TM Kits suited for transfecting primary cells in the 20 µL 16-well … Nettet17. sep. 2024 · Before you begin. This protocol outlines the steps for successful knockdown of LINE1 in either mouse embryos (zygote and 2-cell) or ESCs, followed by RNA FISH validation. The main steps of the ESC protocol can be adapted for other cell types, with the use of different kits for nucleofection (available at Lonza) in order to … Nettet4. sep. 2009 · The POU5F1 gene belongs to the POU (Pit-Oct-Unc) family of transcription factors, that encodes the octamer-binding transcription factor-4 (Oct4) [].In mouse, before the zygotic gene activation the active POU5F1 mRNA is present in the oocyte. The zygotic expression is activated around the 4-cell stage and later restricted to the pluripotent … revoice greg johnson